生命科学文献软件杂项two hybrid.pptx

Two-Hybrid Vector Systems;Protein-Protein Interactions;Functional Genomics;Protein-Protein Interaction Methods;Two-Hybrid Applications;Two-Hybrid Systems;Basics of Two-Hybrid Systems;;GAL4 / LexA Considerations;Limitations of GAL4 / LexA;HybriZAP Vector System;Advantages of l AD Vector;a) Co-infection of XL1-Blue, SupE E.coli cells containing F’ episome coding for F pilus and bearing LamB receptors.;Yeast YRG-2 Reporter Genes;Frozen Competent Yeast Cells;Stratagene’s GAL4 System;Two-Hybrid Products (GAL4);CytoTrap Two-Hybrid Systemfor the Detection of Protein-Protein Interactions;CytoTrap customer;Ras Activation;CDC25H-2 Yeast Host;;;;;;CytoTrap products:;CytoTrap products:;Directional cloning / or random Eco RI cloning of cDNA Exceptional premades available now Brochure available The CytoTrap system is the first is transcriptional activation independent yeast two-hybrid system;Competition;Mammalian Two-Hybrid System;Why Mammalian?;;pGal4BD NF-kB # 478;;;A;Competition;Questions?