人工分子伴侣辅助鸡白细胞介素18重组蛋白的复性.docVIP

人工分子伴侣辅助鸡白细胞介素18重组蛋白的复性 文档信息 属性： Doc-03VRJ6，doc格式，正文15535字。质优实惠，欢迎下载！ 说明： 作为医药学资料、临床医学资料的写作参考资料，提供解决怎么写及格式等相关问题。 适用： 作为文章写作的参考文献，解决如何写好实用应用文、正确编写文案格式、内容摘取等相关工作。 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：人工分子伴侣辅助鸡白细胞介素18重组蛋白的复性 2 1 材料和方法 3 2 结果 6 3 讨论 8 文2：人工分子伴侣辅助鸡白细胞介素18重组蛋白的复性 10 1 材料和方法 11 2 结果 14 3 讨论 17 参考文摘引言： 18 原创性声明（模板） 20 文章致谢（模板） 20 正文 人工分子伴侣辅助鸡白细胞介素18重组蛋白的复性 文1：人工分子伴侣辅助鸡白细胞介素18重组蛋白的复性 ［Abstract］ AIM: To study the technique of boosting the renaturation yield of rChIL-18 by using aritificial molecular chaperone composed of cetyl trimethyl ammonium bromide(CTAB) and β-cyclodextrin(β-CD). METHODS: The recombinant plasmid of mChIL-18 prokaryotic expression was traformed into BL21(DE3) strain and then induced by IPTG at 37℃. The recombinant mChIL-18 was expressed efficiently in inclusion bodies in . After crushed and washed， the inclusion bodies were thoroughly denatured with 6 mol/L of guanidine hydrochloride， and then the artificial molecular chaperone was used to promote protein refolding. After the rehabilitation of products was purified by bag filter， its activity was detected by lymphocyte proliferation assays. RESULTS: The SDSanalysis indicated the expressed ChIL-18 protein had molecular weight of 44 000. The expressed product existed in the form of inclusion protein bands of Mr 44 000 and 26 000 appeared on SDSgel. The percentage of renaturation was with artificial molecular results of MTT assay showed the expression of ChIL-18 protein in BL21(DE3) greatly induced the proliferation of chicken T lymphocytes. CONCLUSION: The artificial chaperone technique can obviously boost the renaturation yield of rChIL-18. The purified and expressed product of fusion chicken Interleukin-18 gene in have relativity high bioactivity。 ［Keywords］artificial molecular chaperone; chicken IL-18 recombination protein; β-cyclodextrin; refolding; surfactant 白细胞介素18（interleukin-18， IL-18）也称为γ干扰素（IFN-γ）诱导因子（interferonγ-inducing factor， IG