番荔枝内酯对人淋巴瘤Raji细胞体外增殖和凋亡的影响.doc

番荔枝内酯对人淋巴瘤Raji细胞体外增殖和凋亡的影响 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：番荔枝内酯对人淋巴瘤Raji细胞体外增殖和凋亡的影响 1 1 实验材料 3 2 实验方法 3 3 统计学方法 4 4 结果 5 5 讨论 6 文2：酒石酸锑钾对人胃癌细胞体外增殖和凋亡的影响 8 1材料和方法 8 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 13 正文 番荔枝内酯对人淋巴瘤Raji细胞体外增殖和凋亡的影响 文1：番荔枝内酯对人淋巴瘤Raji细胞体外增殖和凋亡的影响 Abstract：Objective To investigate the effects of annonaceous acetogenin on proliferation and apoptosis in Raji cells and its mechanism. Methods Raji cells cultured in vitro were pided into control group， annonaceous acetogenin group and adriamycin group. Raji cells were effected by ， ， 25， 50 μg/mL annonaceous acetogenin. Proliferation of Raji cells were evaluated by MTT assays， apoptosis percentage was assessed by flow cytometry. Caspase-9 protein was detected by immunohistochemistry. Results The Raji cell proliferation rate of annonaceous acetogenin decreased compared with the control， that of 25， 50 μg/mL group were lower than adriamycin group， and it was related to the concentration， relying on the incubating time. The apoptosis rate was higher than control， that of 25， 50 μg/mL group were higher than adriamycin group， and it was related to the concentration and the incubating time. The expression of caspase-9 protein of annonaceous acetogenin group was higher than control， and it had a positively relatiohip with the concentration and incubating time. Conclusio Annonaceous acetogenin could inhibit cell proliferation in a dose-dependent and time-dependent manner in Raji cells， and it may induce Raji cells apoptosis by up-regulating caspase-9 expression。 Key words：annonaceous acetogenin；antitumor；apoptosis；proliferation；caspase-9；Raji cell 番荔枝(Annona squamosa Linn.)为番荔枝科番荔枝属植物，我国广东、广西、海南、福建、云南等省及台湾地区均有栽培，东南亚、南美国家及地区也有广泛分布。番荔枝内酯(annonaceous acetogeni)分布在番荔枝科多种植物的根、树皮、种子、叶以及果实中。研究表明番荔枝内酯是一类很有希望的新抗癌药物。笔者研究番荔枝内酯在淋巴瘤治疗中的应用，现将体外实验结果报道如下。 1 实验材料 药物 番荔枝内酯类化合物：为本研究组参照Dos Santos等[1]方法从番荔枝种子中提取分离所得，为白色蜡状粉末。使用前以少许无水乙醇溶解，加生理盐水配制成所需浓度，乙醇终浓度不超过1%；盐酸阿霉素为意大利法玛西亚公司产品。 主要试剂 四甲基偶