植物免疫系统.ppt

The plant immune system;.;MAMPS or PAMPs：microbial- or pathogen-associated molecular patterns PTI:PAMP-triggered immunity PRRs: pattern recognition receptors ETS:effector-triggered susceptibility ETI: effector-triggered immunity HR: hypersensitive cell death response;MAMPS or PAMPs：microbial- or pathogen-associated molecular patterns PTI:PAMP-triggered immunity PRRs: pattern recognition receptors ETS:effector-triggered susceptibility ETI: effector-triggered immunity HR: hypersensitive cell death response;MAMPS or PAMPs：microbial- or pathogen-associated molecular patterns PTI:PAMP-triggered immunity PRRs: pattern recognition receptors ETS:effector-triggered susceptibility ETI: effector-triggered immunity HR: hypersensitive cell death response;Plant immune system activation by pathogen effectors that generate modified self molecular patterns;Co-evolution of host R genes and the pathogen effector complement;.;1.图位克隆法 2.转座子标签法 3.同源序列法;定位克隆技术是根据目标基因在染色体上的位置进行基因克隆的一种方法,目标基因精确定位在染色体特定位置之后,用目标基因两侧紧密连锁的标记筛选含有大的插入片段的基因组文库(如BCA和YA)C,通过染色体步行(ChormosomeWalkign)筛选到含有目标基因的克隆,最后通过遗传转化和功能互补实验进行验证。其核心任务是染色体步行,如果能找到与目标基因很近的标记,以至于二者之间的距离小于基因组文库中克隆的平均插入片段大小,就可以直接筛选到含有目标基因的克隆,最终得到候选基因,这种策略称为染色体登陆(ChromosomeLandign)。该方法的关键是需要高密度的分子标记图谱和找到与目标基因紧密连锁的分子标记。 ;.;.;.;.;此课件下载可自行编辑修改，供参考！ 感谢您的支持，我们努力做得更好！