高级病生动脉硬化讲座硕士班.pptxVIP

“Chronic inflammatory disorder of intima of large blood vessels characterised by formation of fibrofatty plaques called atheroma”. Hardening of arteries - Arteriosclerosis;Macrophages T-lymphocytes ;Atherosclerosis; Lipid metabolism imbalance;Classification of lipids and lipoproteins;Characteristics of lipoproteins;Digestion and metabolism of dietary fat;HDL metabolism and reverse cholesterol transport;Cholesterol efflux and reverse cholesteroltransport is modulated by two receptors;PPARα activators induce cholesterol effluxand reverse cholesterol transport;HDL metabolism:5 key genes ;PPARα: apo A-l, apo A-ll, LPL, ABCA-1 and SR-BI expression;PPARa activators induce cholesterol effluxfrom human macrophages;CLA-1/SR-BI protein may promote cholesterolremoval from peripheral cells;CLA-1 expression is regulated by PPARα activatorsin differentiated human macrophages;PPARα activators induce cholesterol effluxand reverse cholesterol transport;LDL;The early lesion: modified lipoproteins and foam cell formation;Classes of Scavenger Receptors;The Scavenger Receptor Families for modified LDL;第21页/共58页;The macrophage as an inflammatory mediator;Statins induce PPARα activity;Macrophages T-lymphocytes ; Inflammation;Role of macrophages;;TLR receptors and atherosclerosis;;;;;;; Immuno-response;Reduced atherosclerosis in MyD88 null mice;TLRs and atherosclerosis;第38页/共58页;第39页/共58页;第40页/共58页;Why does oxidized LDL become immunogenic?;第42页/共58页;How Does Immunization With Oxidized LDL Affect Atherosclerosis?;Is it possible to produce a vaccine against atherosclerosis?;Characterization of epitopes in oxidized LDL by a library of peptide ELISAs;Active immunization of apo E null mice with apo B-100 peptide sequences;Effect of oxidized LDL peptide vaccines on atherosclerosis in mice;Effect of immunization with MDA-apoB- 100 peptide 45 on specific IgM, IgG, IgG1 (Th2) and IgG2a (Th1) in apo E null mice;Immunization using MDA-apo B-100 peptides in mice;Passive immunization strategy;