人肺鳞癌及肺炎性假瘤组织的蛋白质差异表达谱的初步建立（论文资料）.docVIP

人肺鳞癌及肺炎性假瘤组织的蛋白质差异表达谱的初步建立（论文资料） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：人肺鳞癌及肺炎性假瘤组织的蛋白质差异表达谱的初步建立 1 1 材料与方法 3 2 结果 7 3 讨论 8 文2：人肺鳞癌及肺炎性假瘤组织的蛋白质差异表达谱的初步建立 10 1 材料与方法 12 参考文摘引言： 14 原创性声明（模板） 15 文章致谢（模板） 15 正文 人肺鳞癌及肺炎性假瘤组织的蛋白质差异表达谱的初步建立（论文资料） 文1：人肺鳞癌及肺炎性假瘤组织的蛋白质差异表达谱的初步建立 establishing and analyzing differential proteomic expression profiles from human lung squamous carcinoma and inflammatory pseudotumor tissues ［abstract］ objective to establish differential proteomic expression analysis of high resolution and reproducibility twodimeional electrophoresis profiles from human lung squamous carcinoma tissues and inflammatory pseudotumor the proteome of human lung squamous carcinoma tissues and inflammatory pseudotumor tissues were separated and identified by using a series of methods，including immobilized ph gradient twodimeional polyacrylamide gel electrophoresis，silver staining，imagemaster 2de analysis software，peptide mass fingerprint based on matrixassisted laser desorptioionization time of flight mass spectrometry (malditofms) and database (1) the good 2de pattern of human lung squamous carcinoma tissues and inflammatory pseudotumor tissue including resolution and reproducibility were staining，the 2de image analysis by imagemaster 2de software had detected average of 1569±78 and 1487±64 spots，1454±37 and 1370±43 spots were matched average matching rate were % and %.there had a good reproducibility of spot position in the human lung squamous carcinoma tissues maps，with average deviation in ief direction of (±)mm，while in sdspage direction it was (±)mm.(2) the map of the tumor tissues was compared with inflammatory pseudotumor tissues，1142±51 spots were three protei were analyzed，some of which have the relatiohip of oncogenes，and othe were the regulation protei，which adjust the cell cycle and signal these data suggest that we cotructed high resolution and reproducibility twodimeional electrophoresis profiles from human lu