Agilent Comprehensive Profiling of Free and Conjugated Estrogens by Capillary Electrophoresis-Time of Flight-Mass Spectrometry 说明书用户手册.PDF

Comprehensive Profiling of Free and Conjugated Estrogens by Capillary Electrophoresis-Time of Flight-Mass Spectrometry Application Note Clinical Authors Introduction Naomi Kuehnbaum and The bioavailability and biological activity of estrogens is tightly regulated by phase I/II metabolic transformation processes associated with hydroxylation, glucuronidation Philip Britz-McKibbin or sulfation that are critical to human health, development and fertility. Increasing Department of Chemistry and Chemical clinical evidence suggests that chronic exposure to various dietary, pharmacological and environmental agents can modulate estrogen metabolism or estrogen receptor Biology McMaster University, expression in cells, thereby contributing to higher risks for cancer development. Hamilton, Canada However, there is lack of a specific, sensitive yet robust methodology for compre- hensive estrogen profiling given the wide dynamic range and chemical diversity of Julie Marr and Dawn Stickle endogenous/exogenous estrogen metabolites present in complex biological fluids, Agilent Technologies, Inc. such as urine [1]. Wilmington, DE Experimental used as the nebulizer gas in ESI and the drying gas for the MS. The system softw