Illumina光纤微珠芯片技术.pptVIP

Key Elements: Decoding Quality Metrics BeadArray Formats Sentrix Array Matrix Sentrix BeadChip Formats Human-6 HumanRef-8 Universal-16 Custom-16 Human-1 BeadArray Formats Sentrix Array Matrix Sentrix BeadChip Formats Human-6 HumanRef-8 Universal-16 Custom-16 Human-1 GoldenGateTM Genotyping and DASLTM “Universal Array” BeadArray Formats Sentrix Array Matrix Sentrix BeadChip Formats Human-6 HumanRef-8 Universal-16 Custom-16 Human-1 Focused GEX Sets(Standard Samplers, Custom BeadPools) BeadArray Formats Sentrix Array Matrix Sentrix BeadChip Formats Human-6 HumanRef-8 Universal-16 Custom-16 Human-1 Whole Genome Gene Expression BeadArray Formats Sentrix Array Matrix Sentrix BeadChip Formats Human-6 HumanRef-8 Universal-16 Custom-16 Infinium InfiniumTM Whole Genome Genotyping BeadStation?扫描系统 激光共聚焦扫描系统 高分辨率, 0.8 micron 双色激光 at 532 635 nm 光纤微珠芯片---技术原理 超高分辨率 全自动化定位,扫描 BeadArray Reader Confocal laser scanning system Sub-micron, 0.8 micron Two lasers 532, 635 nm Supports Cy3 Cy5 imaging Auto-focusing, centering and tilting without photo bleaching Supports genotyping, gene expression applications Scans both Array Matrix and BeadChip microarrays Sentrix Bead Chip: Analytical Fluorescent Image User Supplied Dual-block 96-well thermal cycler w/heated lid 2 plate centrifuges (pre and post-PCR) pipette Illumina 提供 BeadArray Reader PC Hybridization chamber Raised bar magnet Heat blocks Oven capable of 45/70oC Plate vortex User documentation BeadStudio　software Installation training Illumina’s BeadStation 500 System 灵活性 扩展性 重复性 均一性 ？？？ 特点 谢谢观看/欢迎下载 BY FAITH I MEAN A VISION OF GOOD ONE CHERISHES AND THE ENTHUSIASM THAT PUSHES ONE TO SEEK ITS FULFILLMENT REGARDLESS OF OBSTACLES. BY FAITH I BY FAITH * Illumina BeadArray? Technology  Application ?Illumina光纤微珠芯片技术及应用? 邱亚静 内容 第一部分：光纤微珠芯片技术 第二部分：基因表达研究 第三部分：基因分型SNP研究 第四部分：最新技术 基因芯片的基本原理 基因芯片又称DNA微阵列（microarray），把大量已知序列探针集成在一个基片上，然后与标记的样品进行杂交，通过检测杂交信号来检测信息 * * * * * 基因芯片的制作 点样法 原位合成 基因芯