常用荧光染料的激发及发射波长.docVIP

常用荧光染料的激发及发射波长 荧光素名称 激发波长 (nm) 发射波长 (nm) 备注 1,5 IAEDANS 336 490 ? 1,8-ANS 372 480 ? 4-Methylumbelliferone 385 502 ? 5-carboxy-2,7-dichlorofluorescein 504 529 ? 5-Carboxyfluorescein (5-FAM) 492 518 ? 5-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH 5-Carboxytetramethylrhodamine (5-TAMRA) 542 568 ? 5-FAM (5-Carboxyfluorescein) 492 518 ? 5-HAT (Hydroxy Tryptamine) 370-415 520-540 ? 5-Hydroxy Tryptamine (HAT) 370-415 520-540 ? 5-ROX (carboxy-X-rhodamine) 578567 604591 ? 5-TAMRA (5-Carboxytetramethylrhodamine) 548542 552568 ? 6-Carboxyrhodamine 6G 518 543 ? 6-CR 6G 518 543 ?