医学免疫学课件：NA甲基化与系统性红斑狼疮.pptxVIP

;Contents;Systematic Lupus Erythematosus ;DNA Methylation;Wu, H., Zhang, A. Y. (2014). Reversing dna methylation: mechanisms, genomics, and biological functions. Cell, 156(1-2), 45-68.;A: PCR results showed that in CD4+ T lymphocytes from SLE patients, DNMT1 is significantly lower than that in normal population (P 0.05); B: Western Blot results showed that in CD4+ T lymphocytes from SLE patients, the protein expression of DNMT1 was significantly lower than that in normal population; C: The DNA total methylation level of CD4+ T lymphocyte in SLE patients were significantly lower than that in healthy people; D: In SLE patients, the expression of DNMT1 and IgG antibody titers were negatively correlated (P 0.05).;Liang, J., Zhu, X. H., Qin, H. H., Lin, J. R., Wang, D. Q., Huang, L., et al. (2015). A correlation study on the effects of DNMT1 on methylation levels in CD4(+) T cells of SLE patients. International Journal of Clinical Experimental Medicine, 8(10), 19701-19708.;A: PCR results showed that, after the transfection of plenti6.3/v5-DNMT1 viral vector into CD4+ T lymphocytes, the mRNA level of DNMT1 were markedly elevated; B: Western Blot results showed that, after the transfection of plenti6.3/v5-DNMT1 viral vector into CD4+ T lymphocytes, the protein level of DNMT1 were markedly elevated; C: Flow cytometry results showed that, after the transfection of plenti6.3/v5-DNMT1 viral vector into CD4+ T lymphocytes, the methylation level of total DNA raised; D: ELISA results show that, compared with the control group, the production of IgG antibody was reduced after the transfection of plenti6.3/v5-DNMT1 viral vector into CD4+ T lymphocytes (P = 0.021); E: 5-Aza-dc cell demethylation can significantly reverse the level of IgG antibody titers in CD4+ T lymphocytes.;Correlation between whole methylation and DNMT1 mRNA in patients with SLE;;CREMα;Rauen, T. (2013). Camp responsive element modulator: a critical regulator of cytokine production. Trends in Molecular Medici