辛伐他汀对糖尿病大鼠肾脏活化蛋白1表达的影响（临床医学范文）.docVIP

辛伐他汀对糖尿病大鼠肾脏活化蛋白1表达的影响（临床医学范文） 目录 TOC \o 1-9 \h \z \u 目录 1 正文 1 文1：辛伐他汀对糖尿病大鼠肾脏活化蛋白1表达的影响 2 1 材料与方法 3 1.1 材料 3 1.2 方法 3 1.2.1 糖尿病大鼠模型 3 1.2.2 动物分组及给药 4 1.2.3 标本的收集及处理 4 1.2.6 统计学分析 5 2 结果 5 文2：辛伐他汀对糖尿病大鼠肾脏活化蛋白1表达的影响 5 1 材料与方法 7 1.1 材料 7 1.2 方法 7 2 结果 9 2.2 24 h尿蛋白、Cr、Glu、TC、TG 9 2.3 大鼠肾组织病理学改变 9 3 讨论 10 参考文摘引言： 11 原创性声明（模板） 13 文章致谢（模板） 14 正文 辛伐他汀对糖尿病大鼠肾脏活化蛋白1表达的影响（临床医学范文） 文1：辛伐他汀对糖尿病大鼠肾脏活化蛋白1表达的影响 ［Abstract］ Objective: To study the molecular mechanism of activator protein1(AP1) in renal tissues of diabetic rats, and to investigate the effects of simvastatin on AP1 and its protection to kidney during the development of diabetic nephropathy (DN). Methods: SD male rats were randomly divided into three groups: normal control group (C), diabetic group (DM), and simvastatin treated group (DS).The expression of AP1（cjun mRNA）in renal tissues was measured with in situ hybridization.The expressio of macrophage inflammatory protein1α (MIP1α) and fibronectin (FN) were measured with Immuno histochemstry method. The levels of triglyceride (TG), total cholesterol (TC), creatinine(Cr) and 24hour urine protein excretion were measured with biochemistry methods. Results: The expressio of AP1, MIP1α and FN in glomeruli were increased in group DM and were inhibited by simvastatin treatment(P0.01). The levels of 24hour urine protein, TG,TC and Cr in group DS were lower than those in group DM(P0.01). The renal histology changes were markedly relieved in group DS as compared with that in group DM. Conclusio: AP1 plays an important role in the development of diabetic nephropathy. One of Renoprotective effects of simvastatin might be inhibition of AP1 activity. ［Key words］ diabetic nephropathies; activato protein1; macrophage inflammatory protein1; cystati 糖尿病肾病（diabetic nephropathy，DN）的发病机理及影响因素比较复杂，至今尚不完全清楚，目前认为糖代谢紊乱、肾局部血流动力学改变、细胞因子等因素均发挥着重要的作用[1]。活化蛋白-1（AP1)是一种转录因子，许多与肾脏疾病进展有关的细胞因子，如转化生长因子β1（TGFβ1）