实时荧光定量PCR技术在实验教学中的应用(1).pdfVIP

实时荧光定量PCR技术在实验教学中的应用 赵玉红;李欣;赵立青;李小菊;石建党;李登文;张金红;周浩 【摘要】The experiment on the real-time fluorescence quantitative PCR(polymerase chain reaction)analysis of the heterologous expression of EGFP gene induced by IPTG in Escherichia coli,is designed,and the effect of IPTG induction concentration and temperature on the expression of EGFP gene is studied.The preliminary results show that under the conditions that the concentration of IPTG is 270 μmol/L,the induction temperature is 16 ℃ and the induction time is 18 h,the expression amount of EGFP mRNA is maximal. Through the study