Gol-NTR-DataSheet-生命科学试剂-MCE.pdf

Hotline: 400-820-3792 Inhibitors • Screening Libraries • Proteins www.MedChemE Gol-NTR Cat. No.: HY-151537 分⼦式: C₂₄H₁₆F₃N₃O₄ 分⼦量: 467.4 作⽤靶点: Fluorescent Dye 作⽤通路: Others 储存⽅式: Please store the product under the recommended conditions in the Certificate of Analysis. BIOLOGICAL ACTIVITY ⽣物活性 Gol-NTR 是⼀种⾼选择性和⾼灵敏度的⾼尔基体靶向探针。Gol-NTR 是硝基还原酶 (NTR) 激活的，具有可视化 急性肺损伤 (ALI) 和修复功能。Gol-NTR 具有 54.8 ng/mL 的低检测限。Gol-NTR 可⽤于监测和评估脓毒症诱导 的 ALI 治疗反应的研究。储存⽅式：避光。 体外研究 Fluorescent labeling of NTR by Gol-NTR [1] (1) Prepare 1.0 mM Gol-NTR stock solution with DMSO solution. (2) Dilute the stock solution with DMSO solution to prepare 5.0 µM Gol-NTR working solution. (3) Mix 5.0 µM Gol-NTR with 50 µM NADH in PBS buffer (10 mM, pH 7.4) containing 5% DMSO, and then add appropriate NTR. (4) After incubation at 37℃ for 30 min, the spectra was recorded at 405 nm (slit width: dex/dem=5/5 nm). Fluorescence labeling of NTR in A549 cells by Gol-NTR [1] (1) A549 cells were cultured at different oxygen concentrations (1%, 5%, 10%, 15% and 20% O2) for 8 h. (2) A549 cells were washed with phosphate buffered saline (PBS). (3) A549 cells were treated with 5.0 µM Gol-NTR for 1 h.