皮肤被毛检查 皮肤刮片（宠物临床检查技术）.pptx

《皮肤刮片》Skin Scraping授课专业：动物医学 Outline《宠物临床检查技术》课件 PURPOSETo identify mites in or on the skin辨认在皮肤上或皮肤内的螨虫（mites）。《宠物临床检查技术》课件 INDICATIONSAny dog or cat with alopecia, scaling, or pruritus (itchiness)。有脱毛、皮屑或发痒症状的犬或猫。《宠物临床检查技术》课件 CONTRAINDICATIONS AND CONCERNS1. No contraindications无禁忌症2. This is a good test for diagnosing mites but is not as sensitive for other mites. It is important to collect and evaluate numerous samples.这种测试对于毛囊虫（Demodex）相当有效，但对于其他类型的螨虫的敏感度并不高。重点在于收集评估大量的样本。《宠物临床检查技术》课件 POSITIONING AND RESTRAINTAdequate restraint to keep the animal still适当的保定以维持动物不动《宠物临床检查技术》课件 SPECIAL ANATOMY1. The mite you are looking for will determine the optimal site to scrape.您所怀疑的生存位置是最适合的刮片位置。2. Sarcoptic mange mites are most likely to be found on pressure points like hocks and elbows as well as on the ear margins. Most affected dogs are extremely pruritic.疥螨虫最有可能在压力点找到，如跗、肘通以及耳朵边缘等。大多受到感染的狗会极度瘙痒。《宠物临床检查技术》课件 《宠物临床检查技术》课件Drawing showing the most likely locations for sarcoptic mangemites.Alopecia, erythema, and excoriations in a dog with sarcoptic mange. 3. Demodex mites are most likely to be found in lesions on the face and feet in dogs with focal disease, whereas generalized demodicosis can affect any location. Demodex mites are often deep within hair follicles, so the skin should be pinched before scraping for these mites.毛囊虫最有可能在脸上以及脚部出现病灶，在犬会出现点状病灶，然而任何位置都有可能受到全身性毛囊症的侵袭。毛囊囊虫通常会深入毛囊，所以刮片之前应先将皮肤捏紧，增加刮到的机会。《宠物临床检查技术》课件 《宠物临床检查技术》课件Drawing showing most likely locations for demodectic mange mites.Facial erythema, scaling, and crusting in a young bulldog withDemodex. 《宠物临床检查技术》课件Periocular alopecia, erythema, and scaling in a golden retriever with Demodex.Demodex pododermatitis in a dog. EQUIPMENT? Clean glass slides干净的载玻片? Coverslips盖玻片? Mineral oil or glycerin矿物油或甘油? Scalpel blade (use the dull end or dull the sharp edge before use)手术刀片? Microscope: use low-power objective (40×)显微镜：使用低倍物镜(40×)? Scissors to cut long hair in the region to be scraped剪刀以剪除需要刮片皮肤区域的长毛《宠