吉林省粳稻种质萌发期耐碱性和耐盐性综合评价.pdfVIP

吉林省粳稻种质萌发期耐碱性和耐盐性综合评价 冯钟慧;刘晓龙;姜昌杰;梁正伟 【摘要】In order to provide excellent germplasm resources for direct seeding and breeding for saline and alkaline stress tolerance, 60 representative local japonica rice cultivars from Jilin province, China, were evaluated for their tolerance to saline and alkaline stresses at seed germination stage based on the membership degree.The saline and alkaline stresses were simulated by 120 mM NaCl ( pH=6.98) and 30 mM Na2 CO3 ( pH=11.05) solutions respectively, and rice seeds were germinated on filter papers soaked with different stress solutions in a petri-dish (9cm diameter) .Five indices of seed germination including germination potential , germination rate, plumule length, root length and root number were measured under stressed or unstressed control conditions .The results revealed that the correlation of resistance to saline and alkaline reached highly significant level ( r=0.728**) , though there are varietal differ-ences.Relative plumule length, relative root length had good correlations with alkaline tolerance ( rBL=0.757 **, r RL=0.812 **) and saline tolerance (r BL =0.862**, rRL=0.781**), representing a useful set of simple and fast monitoring parameters for assess-ing saline/alkaline tolerance levels for individual rice varieties.Based on the membership degree, the varieties were divided into 6 grades of saline or alkaline tolerance:highly tolerant ( HT) , moderately tolerant ( MT) , tolerant ( T) , susceptible ( S) , moderately susceptible (MS), highly susceptible (HS) .We identified varieties Changbai 21 (HT/HT), Tonghe 835 (HT/HT), Ji Yujing (HT/HT), Changbai1 0 (HT/MT), Jinian 6 (MT/HT), Jinian 10 (MT/MT), Jijing 507 (MT/MT), Jijing 509 (MT/MT), Jijing 806 (MT/MT) and Dongdao 4 (MT/MT) as tolerant to both the saline and alkaline; Tonghe 99 (HT/S) and Tonghe 837nbsp;( MT/S) as tolerant to saline but susceptible to alkaline;Jijing 105 ( MT/S) and Ji Nongda 828 ( MT/S) as tolerant to alk