QPCR方法检测慢病毒滴度.docxVIP

μM in water. BIOLOGICAL TITRATION OF LENTIVECTORS BY QUANTITATIVE PCR (QPCR) Lentivectors ussually contain DNAs coding for proteins other than GFP or lacZ and promoters that are specifically active in various primary cells and tissue. Therefore, the majority of LVs will not be titrable on HCT116 cells by flow cytometry. An alternative method is thus needed to measure the number of copies of LV stably integrated in HCT116 target cells after transduction. This assay, based on qPCR, is unequivocal as it measures the number of LV DNA copies integrated in the target cell genome. Howev