花铃期增温与短期土壤渍水耦合影响棉纤维发育的生理与分.docxVIP

花铃期增温与短期土壤渍水耦合影响棉纤维发育的生理与分 摘要 为研究花铃期增温与短期土壤渍水耦合影响棉纤维发育的生理与分子机制，本研究通过大田条件下的田间试验，选取农业大区内典型的棉花品种作为实验材料，在不同增温和不同土壤渍水处理下，对棉花的生理指标、纤维品质指标以及相关基因表达进行研究。结果表明，增温和土壤渍水的耦合作用会显著影响棉花的生理生化指标和纤维品质指标。增温会导致棉纤维长度变短、强度和韧性下降，同时也会显著降低棉纤维中部分基因的表达水平，包括DREB1、MYB、NAC、bZIP等基因。土壤渍水会对棉花生长和发育造成抑制，导致棉花株高、茎直径、叶面积等生理指标下降，同时也会显著影响纤维品质指标，如降低纤维强度和韧性，增加短纤维率，并显著影响棉纤维相关基因的表达水平，包括FLA、CESA、GhRDL和PaTPK等基因。而增温和土壤渍水的耦合作用还会影响棉纤维细胞壁合成和代谢通路的表达，包括纤维素合成通路、木质素合成通路和脂肪酸代谢通路等。 关键词：花铃期；增温；土壤渍水；棉纤维发育；基因表达 Abstract In order to investigate the physiological and molecular mechanisms of the combined effects of temperature increase during flowering and short-term soil waterlogging on cotton fiber development, we conducted a field experiment using typical cotton varieties in the agricultural region under different temperature and soil waterlogging treatments. We studied the physiological and fiber quality indicators of cotton, as well as the expression of related genes. The results showed that the coupling effect of temperature increase and soil waterlogging would significantly affect the physiological and biochemical indicators and fiber quality indicators of cotton. Temperature increase would cause a decrease in cotton fiber length, strength, and toughness. It would also significantly reduce the expression levels of some genes in cotton fibers, including DREB1, MYB, NAC, bZIP and other genes. Soil waterlogging would inhibit cotton growth and development, leading to a decrease in physiological indicators such as plant height, stem diameter, and leaf area. It would also significantly affect the fiber quality indicators, such as decreasing fiber strength and toughness, increasing short fiber rate, and significantly affecting the expression levels of cotton fiber-related genes, including FLA, CESA, GhRDL, and PaTPK. The coupling of temperature increase and soil waterlogging also affects the expression of cotton fiber cell wall synthesis and metabolic pathways, including cellulose synthesis pathway, lignin synthesis pathway, and