生物分离工程英文.pptxVIP

生物分离工程英文第1页/共166页 Chapter 1 Introduction第2页/共166页 §1.1 Downstream processing in biotechnology第3页/共166页 The producing process of a biotechnological product is termed bioprocess, that can be divided into two processes as followsUpstream processing：strain development and bioreaction （enzyme catalyze reaction, microbial fermentation, plant/mammalian cell culture, etc）Downstream processing：the isolation and purification of biotechnological productsThe complexity of downstream processing is determined by the required purity of the product, in turn determined by its application.第4页/共166页 The downstream processing scheme normally can be divided into the following stagesBrothSolid-liquid separation Extracellular productIntracellular product→↗↘Cell disruptionRemoval of cell debrisPrimary isolationpurificationformulationFinal product→→→↗↘→→→→第5页/共166页 §1.2 Demands on the  downstream processing第6页/共166页 Strictly monitor the DSP steps for keeping the activity of the product;Rapidly remove those impurities can affecting the stability of the final product;Generally it is necessary to apply special efficient methods to the purification of the product;Since many products are applied to food, pharmaceutical, and cosmetics, those substances harmful to mankind health must be removed;Since the product concentration is low in the culture broth, it is necessary to concentrate the broth for removing large amounts of water. Because those of unit operations are costly, the cost of DSP is increased observably. 第7页/共166页 §1.3 Separation mechanism and unit operation 第8页/共166页 According to different separation principles the unit operations can be sorted into two groups：Mechanical separationObject：unhomogeneous phase systemMechanism：separation based on the difference of substances’ size and densityUnit operation：filtration, settling, centrifugation, etc.Mass transfer separationObject：homogeneous phase systemTransport/velocity separationMechanism：separation based on the