生物信息学讲课五20031122考.pptxVIP

生物信息学讲课; For the past 30-40 years, biology at the molecular and cellular level has been studied from the perspective of analyzing individual genes and individual proteins. Systems biology, on the other hand, is interested in analyzing whole systems of genes or proteins. What this means is that we use tools for capturing information from many different elements of the overall system. And we have to be able to integrate the information thats obtained from all the different biological levels--DNA information, RNA information, protein information, protein interaction information, pathways and so forth. The ultimate objective is to use this information to write mathematical models that are capable of predicting something about the structure of the biologic system under evaluation as well as predicting something about its properties, given particular kinds of stimuli or perturbations. ;;;;;microarrays;第8页/共162页;功能图谱;;后基因组时代对功能理解的本质变化;研究思路的变化;更好整合生物过程不同阶段的分散数据. 基因组 + 转录组+ 蛋白质组 + 代谢组 满足复杂查询的整合数据库. 对复杂生物过程的更好模拟. 蛋白质折叠. 复杂系统建模. Signaling/Metabolic pathways. Pathogenesis. 生物过程动态研究. From the components of a pathway to the dynamics of a pathway.;第14页/共162页;（二）、系统生物学研究的一些例子;基因通过复杂的多反馈网络发挥作用;;哺乳动物细胞周期调控网络(部分)。;Annotating the Yeast Genome;;整合转录组和蛋白质组实验数据后获得的精细功能图谱;;E-cell;;Michael B. Elowitz, et al, A synthetic oscillatory network of transcriptional regulators``, Nature , v403, 2000 ;;;Modeling the Heart ---- from Genes to Cells to the Whole OrganSciences Vol 295 1 March 2002;Molecular Networks: The Top-Down View Dennis Bray Science 26 Sep 2003 ;（三）、系统生物学的研究思路;多信息融合构建功能图谱;From Cell, 2001, V0l. 104, 333;系统生物学研究方法的创新点;;第35页/共162页;酵母细胞周期表达谱分析共调控基因;大规模基因表达谱用于基因调控网络构建;（四）、可能的应用;肿瘤研究;肿瘤研究;心血管疾病研究;第42页/共162页;Spectral Analysis of the Protein-protein Interaction Network in Budding yeast;The topological structure in protein-protein interaction network In clique, proteins connect quite tightly, almost interacting with each other. However, in each bipartite, p