贴壁细胞的免疫荧光染色方法.docxVIP

贴壁细胞的免疫荧光染色方法 Materials: PBS solution 4% Paraformaldehyde (PFA fixative): Dissolve 4g paraformaldehyde in 100ml PBS solution, stir at 70 ℃ to dissolve; PBS-T solution: (0.1% Triton X-100 in PBS solution) PBS-B blocking solution: (4% BSA in PBS solution) shouldPrimary antibody: Dilute with PBS-B solution, dilution factors should refer to manual, or, test from 1:50~200, be more concentrate than application in Western blot; should Secondary antibody: Dilute with PBS-B solution, dilution factors should refer to manual Procedure: Cultured cells, let it attach to the coverslips in 6-well plate; Rem