DB37_T 3802-2019花生品种鉴定技术规程　SSR标记法.pdf

ICS 65. 020B 04DB37山東东省地方标准DB37/T3802—2019花生品种鉴定技术规程SSR标记法Protocol for identifying peanut variety—SSR marker method2019-12-24发布2020-01-24实施山东省市场监督管理局发布 DB37/T 3802—2019前言本标准按照GB/T1.12009给出的规则起草，本标准由山东省农业农村厅提出并组织实施本标准由山东省农业标准化技术委员会归口。本标准起草单位：山东省花生研究所。本标准主要起草人：闫彩霞、单世华、赵小波、季者娟、王娟、苑翠玲、孙全喜、宋秀霞I DB37/T 3802—2019附录C(资料性附录）推荐引物推荐引物见表C.1。表C.1推荐引物引物连锁群引物序列（5°→3r常见等位变异（bp)参照品种160zh. h2461162zh. h5075164zh. h2406Ftcggtttgggagacactett166zh. h1961Ah1TC5A06A07Rttgtaagcagacgccacatc1686-604 4z172zh. h2250176zh. h2405180zh. h2764188zh. h0537168zh. h2413174 zh. h2499Fccatgtgaggtatcagtaaagaagg182zh. h5399Ah2TC11H06B04Rccaccaacaacatt ggat gat198zh. h0930202zh. h2405206zh. h2764210zh. h474989zh. h474991Ah3TC23C08B06Fagcagagt ggaaaacgaagag86zh. h2406Rgt cagt ttgt gaat cggg tt tt9919804 *4z101 zh. h1797103zh. h1585150zh. h5060154zh. h2462B10F-catattgacggtgatggeagAHGS0138162zh. h2405Rccgaccctaatcctaatacaaca164 zh. h2682168zh. h5034174 zh. h2069 9 DB37/T 3802—2019表 C.1推荐引物（续）引物连锁群引物序列（5°→3° )常见等位变异（bp）参照品种65zh, h233068zh, h2413Fagcaaatgaaggtgagggtg90zh. h2250AHGS0151A05Rgcccaaaaatgctaacgaag108zh. h2405112 zh. h2591115zh. h5075118zh. h5034233zh. h09492366690SSHV未定位F-agat tggtggtgacagaggc253zh. h2250Rgccatcgatgtaaccctcac260zh. h0525263zh, h2405266zh. h246666zh. h223174zh. h1585GV1843未定位Fcccacacacacacacacacta82 zh. h0531Raggtgatgctgcgtttcttt86zh. h225090zh. h260894zh, h246277zh. h5034Fcatcccatcattttccctctt85zh. h5327GV1996B03Rtacagtgaaggtgggatcctg87zh. h139589zh. h276492zh. h240545zh. h168955 zh. h2702Fatgctctcagttcttgcctga57zh. h2381GV2638A0463zh. h4749Rcagacataacagtcagtttcacc65zh. h532769 zh. h225073 zh. b0949191zh. h5034194 zh, h1602GN357未定位F-aggtttgctttgggatgatg197zh. h2406Rccgataaaaccaggcaagaa607zh. h0537215zh. h0861218zh. h133110 DB37/T 3802—2019表 C.1推荐引物（续）引物连锁群引物序列（5°常见等位变异（bp）参照品种188zh, h2550194 zh, h0540197zh. h2561007zh. h2405GNB317未定位FgaaaagcttgcaaaatcgagaRtccttccatgttggtgaatg203zh. h2764212zh. h5034224zh, h2462227zh. h0537230zh. h474956 zh. h052562 zh. h0934Ftcagggtgggttaccaaca