DB37_T 3104-2018向日葵杂交种品种纯度SSR分子检测标准.pdf

ICS 67. 060B21DB37山 東东省地方标准DB37/T3104——2018向日葵杂交种品种纯度SSR分子检测标准The variety purity identification technique of sunflower seeds with SSR2018-02-02发布2018-03-02实施山东省质量技术监督局发布 DB37/T言本标准按照GB/T1.1-2009给出的规则起草。本标准由山东省农业厅提出。本标准由山东省农业标准化技术委员会归口。本标准主要起草单位：山东农业大学、北京德农种业有限公司。本标准起草人：张者庆、赵洪、郑建朋、温大兴、季岩，本标准为首次发布。I DB37/T 3104—2018表A.1引物及其参数(续)引物名称序列(5’ to3)Tn退火温度GGGCATAGTTTCCTCCGTTT57. 8AAGTTCTCACGGTTCTTACAACTC58. 657 OFS1146FGGCTCATCACTTGCATCTATTGT58. 4OFS1146RTGAAGACACCATCTCCAATGC58. D57 CAAAGTCCCAAACCACAACC57. 8 57 3266540GGGAAACAAGTTCCTTGATGG58. DJS66540CATGCTTTCTAGGATGGTCAGTT58. 43S66540TGTATGTGGAGGCCAACAAGTAT58.457662540TCCAGCAAGTCAGCAACAAC57. 8366250ACTCCCTCCCATTCTCGTCT59. 857 J986580TCCAGTTATCCAAAGGTGTGC58. 0986S40GTGCTAGAAGAACCTGTAACCATT58. 657 OFS1237FTTCCAGAAATTGGAGAGCAAA54. 1OFS1237RGATTCTTAGCAAACGCAGAGG58. D53 (+1)TGTTCATCAGGTTTGTCTCCA56. 1OFS342-R55. 855 CACCAGCATAGCCATTCAAA1S6S40TGAAACGTAGTAACCTGCCAAA56. 33156S40TTGGACGACCTCGGTATCTT57. 855 2ISS0TTGTCTTCATCTGCGTGTGA55. 8TTGCTGTTGTTGATCGTGT55. 855 OFS677-FTCCTTCCTTCATTCTCTGCATT56. 3CTCCATACCGTTGCCATCAT57. 855 OFS1179FGATTCGGAGCTGTTAGGAGGTAG62. DOFS1179RAAACGGGAAGCAAGAATAGAACA56. 656 OTSSIOCATCGCGTCCCTCTCTCTAA59. 8OFS510-RCCAACCATCACAGCAATCAG57. 857 (1)16S40AAGGATCACTGGAAGAACAATGG58. 43116S40TAAACGCTTGTGACGGTTAGTTG58. 457 (1)TCATATGGAGGGATCTGTTGG58. 0OFS423RAAGCAACCATAATGCATCAGAA54. 554 (1)OFS4-FACAAGTCGCTGGTGAGC59. 6ACATGAAACACGAGCTAAACC56. 155OFS810-FTTCAACGTGCGTGATTAAGG55. 8OFS810RGCGATTTCTTTGGAGACGAA55. 855S0TTCCATTTCCACCATTTTGGC56. 1OFS57RCATTCATGGCCTAAAAGGTTC56. 1550601S0CCTTTGATGTAGTTAAGGAAGTTGTG8 85OFS1030RCGATCAATTTATATGACCGAATTACC57. 356 (1) DB37/T 3104—2018表A1引物及其参数(续)引物名称序列(5’ to3)Tn退火温度IESIOTCCCGAATTAGCCAAAGAAC55. 8IVI39V39L191999191998*65554609580GCGAAGGAACTGAACCGATA57.GATTTTAGTCCGCCAATCA55. 855 OFS488-FCCCATTCACTCCTGTTTCCA57. 8OFS488-RCTCCGGTGAGGATTTGGATT57. 857 106SI0CGTGACCTGTGAAACACCAA57. 8310ESI0CGATAACCGTGTGAATCGTG57. 857 JS0TCCACAAGGACCAACTACGA57.8TCCAACTGCACACACTCGAT57. 8 57OF