螺旋藻藻蓝蛋白的自催化重组及藻蓝胆素裂合酶功能比较研究的中期报告.docxVIP

螺旋藻藻蓝蛋白的自催化重组及藻蓝胆素裂合酶功能比较研究的中期报告 摘要： 螺旋藻是一种富含蛋白质和营养素的蓝藻，其藻蓝胆素和藻蓝蛋白是其主要光合色素和光能转换蛋白。本研究利用重组蛋白技术，对螺旋藻藻蓝蛋白的自催化重组和藻蓝胆素裂合酶功能进行了比较研究。通过菌落PCR扩增和限制酶切分析验证了重组蛋白的正确性。在纳米流体传感器上进行了蛋白质互作实验，结果表明，在自催化重组后，螺旋藻藻蓝蛋白的光吸收能力和光催化效率均有所提高。同时，利用荧光法和吸收光谱法对藻蓝胆素裂合酶的活性进行了测定，并将其与未经重组的藻蓝胆素裂合酶进行了比较。结果表明，经过自催化重组的藻蓝胆素裂合酶具有更高的催化效率和电子转移能力。 关键词：螺旋藻；藻蓝胆素裂合酶；藻蓝蛋白；自催化重组；光催化效率 Abstract: Spirulina is a kind of blue-green algae rich in proteins and nutrients. Its phycocyanin and allophycocyanin are the main photosynthetic pigments and energy conversion proteins. In this study, the self-catalytic recombination of spirulina phycocyanin and the comparative study of the function of its phycocyanin lyase were conducted by using recombinant protein technology. The correctness of the recombinant protein was validated by colony PCR amplification and restriction enzyme analysis. Protein interaction experiment was conducted on a nanofluidic sensor, which showed that the light absorption capacity and photocatalytic efficiency of spirulina phycocyanin were improved after self-catalytic recombination. Meanwhile, the activity of phycocyanin lyase was determined by fluorescence and absorption spectroscopy, and was compared with that of un-recombined phycocyanin lyase. The results showed that the phycocyanin lyase after self-catalytic recombination had higher catalytic efficiency and electron transfer capability. Keywords: Spirulina; phycocyanin lyase; phycocyanin; self-catalytic recombination; photocatalytic efficiency