发酵工艺：下游加工过程（英文版）.pptx

Downstream Processing 2Downstream processing, what and whyDownstream processing is any treatment of culture broth after fermentation to concentrate and purify products. Separation Processes The challenge?Low product concentrations?Large number of impurities?Thermolabile bioproducts An ideal bioseparation process should combine high throughput with high selectivity, and should ensure stability of product. Happy growingDrug producinFermentationKill the microbesSmash the microbeRemove cells/deb isConcentrate and Purify the productFormulate productDownstream ProcessingMicrobesGenetic modificationMarketNutrien ts Oxygen Biological productsProductNature of bioseparation requiredAlcoholic beverages: Beer, wine, spiritsClarification, distillationOrganic acids:Acetic acid, citric acidPrecipitation, filtration, adsorptionVitamins:Vitamin C, vitamin B12Precipitation, filtration, adsorptionAmino acids: Lysine, glycine,Precipitation, filtration, adsorptionAntibiotics: PenicillinsPrecipitation, filtration, adsorptionEnzyme,PolysaccharidesFiltration, precipitation, centrifugation, adsorption, chromatography DistillationSeparating components with different volatilities. Such as organic solvents volatile flavors and aromas. Distillation Stages in Downstream ProcessingRemoval of insolublesProduct IsolationProduct PurificationProduct Polishing 1. Cell removal (filtration, centrifugation)Primary isolation (adsorption, precipitatRemoving components with properties significantl different from those of the productsLarge volume, relatively non selective 3. Purification (chromatography, ultrafilt?Removing components with properties similar to those of the products?Highly selective4. Product preparation (crystallization, dr Removal of insolublesCapture of the product as a solute in a particulate-free liquidExampleSeparation of cells, cell debris or other particulate matter from fermentation broth containing an antibiotic. Typical OperationsFiltrationA mechanical operation used for the separa