RNA干扰抑制旋毛虫副肌球蛋白基因功能的实验研究的中期报告.docxVIP

RNA干扰抑制旋毛虫副肌球蛋白基因功能的实验研究的中期报告 【摘要】RNA干扰技术被广泛应用于基因功能研究。本研究旨在通过RNA干扰抑制旋毛虫副肌球蛋白基因的表达来探究其在细胞运动过程中的功能。经过设计、合成、转染筛选和验证，我们成功地获取了具有较高RNA干扰效果的慢病毒载体，并能够成功地将该载体引入到旋毛虫细胞中。目前已完成了对目标基因的RNA干扰处理和对细胞骨架结构、细胞迁移和定位的相关实验，初步结果表明，RNA干扰可以有效地抑制副肌球蛋白基因的表达，并对旋毛虫的细胞运动产生了影响。下一步将进一步明确不同剂量和时间的RNA干扰处理对细胞运动的影响，以及副肌球蛋白在细胞运动中的具体作用机制。 【关键词】RNA干扰；旋毛虫；副肌球蛋白；细胞运动 【Abstract】RNA interference technology has been widely used in gene function research. The aim of this study was to explore the function of actin-related protein in cell motility by suppressing its expression through RNA interference in Leishmania. After design, synthesis, transfection screening and validation, we successfully obtained a slow virus vector with high RNA interference effect and successfully introduced the vector into Leishmania cells. RNA interference treatment of the target gene and related experiments on cell skeleton structure, cell migration, and localization have been completed. Preliminary results show that RNA interference can effectively inhibit the expression of actin-related protein genes and affects cell motility in Leishmania. Next, we will further clarify the effect of RNA interference treatment at different doses and times on cell movement, and the specific mechanism of actin-related protein in cell movement. 【Keywords】RNA interference; Leishmania; Actin-related protein; Cell motility