键合型对氯苯基氨基甲酸酯化纤维素手性固定相的制备及在HPLC的应用的中期报告.docxVIP

键合型对氯苯基氨基甲酸酯化纤维素手性固定相的制备及在HPLC的应用的中期报告 摘要 本中期报告旨在介绍一种新型手性固定相的制备方法及其在高效液相色谱（HPLC）中的应用。首先通过化学修饰对纤维素进行改性，得到氯苯基氨基甲酸酯化纤维素（CBAMC）。然后采用键合法将L-苯丙氨酸（L-PA）引入CBAMC中，最终得到L-PA键合型CBAMC固定相。通过FT-IR、TGA、SEM等测试手段对其结构和性能进行表征。实验结果表明，成功制备出具有手性选择性能的固定相。在HPLC测试中，该固定相对于对映体的拆分度能够达到95%以上，显示出较好的手性分离效果。 关键词：氨基甲酸酯化纤维素；键合型；手性固定相；高效液相色谱 Abstract This midterm report aims to present a novel preparation method for a chiral stationary phase and its application in high-performance liquid chromatography (HPLC). Firstly, the cellulose was chemically modified to obtain chlorobenzylamino methyl cellulose (CBAMC). Then, L-phenylalanine (L-PA) was introduced into CBAMC by the bonding method to obtain L-PA-bonded CBAMC stationary phase. The structure and properties of the stationary phase were characterized by FT-IR, TGA, SEM, and other testing methods. The experimental results show that a stationary phase with chiral selectivity has been successfully prepared. In the HPLC test, the separation degree of the stationary phase for enantiomers can reach more than 95%, showing good chiral separation performance. Keywords: amino methyl cellulose; bonded type; chiral stationary phase; high-performance liquid chromatography