里氏木霉转录因子XYR1的多效性研究及其在产纤维素酶方面的应用的中期报告.docxVIP

里氏木霉转录因子XYR1的多效性研究及其在产纤维素酶方面的应用的中期报告 （中英文对照） 摘要： 里氏木霉是一种重要的真菌，能够产生多种产业上应用广泛的酶类。其转录因子XYR1在木霉的纤维素酶系统中起关键调控作用。本研究对里氏木霉XYR1的多效性进行了深入探究，并研究了其在产纤维素酶方面的应用。 方法： 将里氏木霉的XYR1转录因子在真菌中过量表达，并检测其在纤维素酶产生、糖类代谢和生长等方面对基因表达的调控作用。同时，利用基因敲除技术分析XYR1在产纤维素酶方面的作用机制。 结果： 过量表达XYR1会显著增强里氏木霉的纤维素酶产生，但对糖类代谢和生长的影响不大。XYR1的基因敲除会显著降低纤维素酶的产生。此外，我们还发现XYR1对木聚糖酶和半乳糖酶等多种酶的基因表达也具有调控作用。 结论： XYR1转录因子在里氏木霉的纤维素酶系统中具有多重作用，其过量表达有利于提高纤维素酶的产量。同时，它还参与了其他糖类代谢酶的调控。这些结果有望为利用里氏木霉在生物质降解方面的潜力提供新思路和理论基础。 Abstract: Trichoderma reesei is an important fungus that can produce a variety of enzymes widely used in industry. Its transcription factor XYR1 plays a key regulatory role in the cellulase system of T. reesei. In this study, we investigated the multifunctionality of XYR1 in T. reesei and its application in cellulase production. Methods: The XYR1 transcription factor in T. reesei was overexpressed in fungi, and its regulatory effect on gene expression in cellulase production, carbohydrate metabolism, and growth was analyzed. At the same time, the role of XYR1 in cellulase production was analyzed using gene knockout technology. Results: Overexpression of XYR1 significantly enhanced cellulase production in T. reesei, but had little effect on carbohydrate metabolism and growth. The deletion of XYR1 significantly reduced cellulase production. In addition, we also found that XYR1 plays a regulatory role in the gene expression of various enzymes, such as xylanase and galactose. Conclusion: The XYR1 transcription factor plays multiple roles in the cellulase system of T. reesei, and its overexpression is beneficial to the production of cellulase. At the same time, it also participates in the regulation of other carbohydrate metabolism enzymes. These results may provide new ideas and theoretical basis for utilizing the potential of T. reesei in biomass degradation.