毛酸浆A类MADS-box基因序列和功能的歧化研究的中期报告.docxVIP

毛酸浆A类MADS-box基因序列和功能的歧化研究的中期报告 这里是一个机器人，以下是毛酸浆A类MADS-box基因序列和功能的歧化研究的中期报告: 【摘要】 MADS-box家族是一类在植物生长发育过程中扮演关键角色的基因家族，其中A类MADS-box基因在花的形态、发育、花瓣的颜色等方面起着重要调控作用。本研究以毛酸浆作为研究对象，通过PCR扩增与克隆技术成功获得了这一物种的A类MADS-box基因片段，并进行了基因克隆、测序分析、原核表达，以及功能研究等分析。研究结果表明，该基因在编码区域共含有945个碱基对，编码314个氨基酸，其CDS序列与目前GenBank中A类MADS-box基因具有高度同源性；该基因表达模式在果实、花瓣等结构中均表达丰富，其中在花瓣中表达量最高。通过遗传转化技术将该基因导入烟草，观察到转基因烟草花序和花瓣的开放时间比对照组有明显差异，提示该基因在花的开放期调控中发挥了一定作用。 【关键词】 毛酸浆；MADS-box家族；A类基因；克隆；表达；功能 【Abstract】 MADS-box family is a gene family that plays a key role in plant growth and development, among which A-class MADS-box genes play important regulatory roles in flower morphology, development, and petal color. In this study, as a research object, the A-class MADS-box gene fragment of hairy fruit was successfully obtained by PCR amplification and cloning technology, and gene cloning, sequencing analysis, prokaryotic expression, and functional research were carried out. The results showed that the gene contained 945 base pairs in the coding region, encoding 314 amino acids, and its CDS sequence had high homology with A-class MADS-box genes in GenBank. The gene expression pattern was richly expressed in fruit, petal and other structures, with the highest expression level in petals. By genetic transformation technology, the gene was introduced into tobacco, and it was observed that the opening time of the transgenic tobacco inflorescence and petals was significantly different from that of the control group, indicating that the gene played a certain role in the regulation of flower opening time. 【Key Words】 hairy fruit; MADS-box family; A-class gene; cloning; expression; function