小鼠甲状腺过氧化物Phe473-Ala800多肽的表达与纯化的中期报告.docxVIP

小鼠甲状腺过氧化物Phe473-Ala800多肽的表达与纯化的中期报告 （英文版） Expression and purification of a mouse thyroid peroxidase Phe473-Ala800 peptide: mid-term report Thyroid peroxidase (TPO) is a key enzyme in the biosynthesis of thyroid hormones. The Phe473-Ala800 peptide of mouse TPO contains the majority of the TPO antigenic epitopes recognized by autoantibodies in autoimmune thyroid diseases. In this report, we describe the mid-term progress of expressing and purifying this peptide. The Phe473-Ala800 peptide was cloned into a pET-28a(+) vector and expressed in BL21(DE3) Escherichia coli cells. After induction with 0