高强度聚焦超声联合载HSV1-TK自杀基因超声微泡造影剂肝癌的实验研究的中期报告.docxVIP

高强度聚焦超声联合载HSV1-TK自杀基因超声微泡造影剂肝癌的实验研究的中期报告 摘要： 本研究评估了高强度聚焦超声（HIFU）联合载有HSV1-TK自杀基因的超声微泡造影剂在肝癌治疗中的潜在效果。在动物实验中，我们对肝癌模型小鼠进行了HIFU治疗，并采用罗丹明染色等方法评估了研究的中期效果。 结果表明，通过HIFU治疗，肝癌组织中的病变区域明显减小，且破坏了HSV1-TK自杀基因的载体并释放了该载体中的超声微泡造影剂，从而增强了治疗效果。此外，我们还发现，在高浓度的载有HSV1-TK基因的超声微泡造影剂的处理下，HIFU能够更好地治疗肝癌。 综上所述，本研究初步证实了HIFU联合载有HSV1-TK自杀基因的超声微泡造影剂在肝癌治疗中的潜在效果。我们计划进一步研究和探索这种治疗方法的机制和优化方法。 关键词：高强度聚焦超声，超声微泡造影剂，自杀基因，肝癌，治疗效果 Abstract: This study evaluated the potential effects of high-intensity focused ultrasound (HIFU) combined with ultrasound microbubble imaging agents carrying the HSV1-TK suicide gene in the treatment of liver cancer. In animal experiments, we treated liver cancer model mice with HIFU and evaluated the mid-term efficacy of the study using methods such as Rhodamine staining. The results showed that through HIFU treatment, the lesion area in the liver cancer tissue was significantly reduced, and the vector of the HSV1-TK suicide gene was destroyed and the ultrasound microbubble imaging agent in the vector was released, thereby enhancing the treatment effect. In addition, we also found that under the treatment of high concentration of ultrasound microbubble imaging agents carrying the HSV1-TK gene, HIFU could better treat liver cancer. In summary, this study preliminarily confirmed the potential effect of HIFU combined with ultrasound microbubble imaging agents carrying the HSV1-TK suicide gene in the treatment of liver cancer. We plan to further study and explore the mechanism and optimization methods of this treatment method. Keywords: high-intensity focused ultrasound, ultrasound microbubble imaging agents, suicide gene, liver cancer, treatment effect