大鼠触液核TRPV1的分布及其在神经病理性痛条件下的表达的中期报告.docxVIP

大鼠触液核TRPV1的分布及其在神经病理性痛条件下的表达的中期报告 摘要： 触液核是大脑中一种特殊的感觉核，参与了疼痛调节、情感和记忆等功能。目前尚不清楚触液核中TRPV1的表达情况以及其在神经病理性痛中的作用。本实验使用免疫荧光染色和Western blotting方法研究了大鼠触液核中TRPV1的分布和表达情况，并采用行为学方法观察了TRPV1与神经病理性痛的关系。实验结果显示：TRPV1在大鼠触液核表达最强，主要分布于触液核的外侧和中心区域；在神经病理性痛条件下，TRPV1的表达明显上调，并且与痛觉敏感度呈正相关。这些结果表明，TRPV1在大鼠触液核中发挥了重要的作用，可能是神经病理性痛的重要机制之一。 关键词: 触液核, TRPV1, 神经病理性痛, 分布, 表达 Abstract: The trigeminal subnucleus is a specific sensory nucleus in the brain that plays a role in pain modulation, emotions and memory. However, it is still unclear whether TRPV1 is expressed in the trigeminal subnucleus and its role in neuropathic pain. In this study, we used immunofluorescence staining and Western blotting methods to investigate the distribution and expression of TRPV1 in the trigeminal subnucleus of rats, and observed the relationship between TRPV1 and neuropathic pain using behavioral methods. The results showed that TRPV1 was most strongly expressed in the trigeminal subnucleus of rats, mainly distributed in the lateral and central areas of the subnucleus; under neuropathic pain conditions, TRPV1 expression was up-regulated and positively correlated with pain sensitivity. These results indicate that TRPV1 plays an important role in the trigeminal subnucleus of rats and may be one of the important mechanisms of neuropathic pain. Keywords: trigeminal subnucleus, TRPV1, neuropathic pain, distribution, expression