华蟾素注射液对人肝癌HepG-2细胞增殖、凋亡及周期的影响的中期报告.docxVIP

华蟾素注射液对人肝癌HepG-2细胞增殖、凋亡及周期的影响的中期报告 中文摘要：本研究探究华蟾素注射液对人肝癌HepG-2细胞增殖、凋亡及周期的影响。采用MTT法检测细胞增殖能力，Annexin V-FITC/PI染色及流式细胞仪检测细胞凋亡，PI染色及流式细胞仪检测细胞周期。结果显示，华蟾素注射液对HepG-2细胞有明显的抑制作用，能够抑制细胞增殖并促进细胞凋亡，同时也能够导致细胞周期的停滞。本研究初步证明了华蟾素注射液具有抗肝癌的潜力，为其临床应用提供了一定的科学依据。 英文摘要：This study investigated the effect of Huachansu injection on proliferation, apoptosis, and cell cycle of human liver cancer HepG-2 cells. MTT assay was used to detect cell proliferation, Annexin V-FITC/PI staining and flow cytometry were used to detect cell apoptosis, and PI staining and flow cytometry were used to detect cell cycle. The results showed that Huachansu injection had a significant inhibitory effect on HepG-2 cells, evidenced by reduced cell proliferation, increased cell apoptosis, and cell cycle arrest. This study provides preliminary evidence for the potential of Huachansu injection as an anti-liver cancer drug, which may offer a scientific basis for its clinical application.