英文细胞分裂实验报告.docVIP

生物系实验报告 （Prohibited?for commercial purposes） 姓名 班级 学号 实验日期 科目 实验名称 the cell cycle and mitosis 合作者 指导教师 成绩 LAB 6: The Cell Cycle and Mitosis Introduction: Mitosis is the main process of cellular reproduction. It contains interphase, metaphase, anaphase and telophase; cytokinesis included. Two identical daughter cells which nearly possess the same genetic materials in parent cell. Interphase is a phase including G1, S, and G2, which is not part of mitosis. The chromosomal materials whose DNA have been fixated can be dyed by Feulgen stain. According to the root tips cells we observe, the different mitotic stages can be easily distinguished. In this lab, we can observe different stages of mitosis in plants cells. We will make a wet mount slide of garlic root tips, so that we can learn to use selective staining techniques (only stain DNA in chromosomes)- Feulgen stain. Materials and methods: Materials: Compound microscope (NIKON ECLIPSE E100), water bath (at 60℃), glacial acetic acid-methanol fixative (1:3), 1M hydrochloric acid, 45% acetic acid, Feulgen stain, prepared slides of onion root tip, lens tissue, garlic root tips, forceps, slides, cover slips. Methods: Part Ⅰ： ①Observe a prepared slide of a longitudinal section of onion root tip with microscope, from low-power to high-power. ②Concentrate in the region where the apical meristem of the root, and identify cells in different phases. ③Look at 30-50 onion root tip cells, then tally the frequency of mitotic phases. Record the data in table T-1. Table T-1: (based on Figure 1-1.1) Determining duration of cell cycle Phase Number seen % of Total Interphase 39 79.6 Mitotic 10 20.4 Total 49 100 Part Ⅱ： In order to prepare