大型溞胆碱酯酶基因工程抗原的制备及其抗体的适用性研究的中期报告.docxVIP

大型溞胆碱酯酶基因工程抗原的制备及其抗体的适用性研究的中期报告 摘要： 溞胆碱酯酶（AChE）是一种重要的酶，它在生物体内起到调节神经传递的作用。因此，AChE抗体具有重要的生物医学研究价值。本研究利用基因工程技术成功制备了大型AChE抗原，并通过Western blot和ELISA等方法验证了其抗原性。同时，利用制备的AChE抗原成功制备了AChE多克隆抗体。经过免疫组化实验和ELISA测试，发现该抗体在检测AChE的表达和活性方面具有很好的适用性。未来，将应用该多克隆抗体进行更深入的生物医学研究。 关键词：溞胆碱酯酶，抗原，抗体，基因工程，生物医学 Abstract: Acetylcholinesterase (AChE) is an important enzyme that regulates neural transmission in living organisms. Therefore, AChE antibodies have important biomedical research value. In this study, a large-scale AChE antigen was successfully prepared using genetic engineering technology, and its antigenicity was verified by Western blot and ELISA. At the same time, AChE polyclonal antibodies were successfully prepared using the prepared AChE antigen. Through immunohistochemistry and ELISA tests, it was found that the antibody has good applicability in detecting AChE expression and activity. In the future, this polyclonal antibody will be used for further biomedical research. Keywords: Acetylcholinesterase, antigen, antibody, genetic engineering, biomedical