机能实验报告英文版影响尿生成的因素.doc

Function experiment report Regulation of Urine Formation 本组承诺：本实验报告原创，绝无抄袭！ 组员： Influence factors of urine formation [PURPOSE]: Master influence factors of urine formation and analyzes the mechanism, further understand the renal excretory function. [ANIMAL]: Rabbit [METHODS]: Operation Take one rabbit, weighing. According to 4ml/kg, find the vine at the edge of an ear and tnen inject into the 25% urethane solution for anesthesia. Observe the respiratoryrate, corneal reflection, skin clip kneading and muscle relaxation. Operation in cervix: Separate the right vagus nerve and thread a period of line under it. Set aside. Operation in abdomen: Firstly, ligate the external orifice of urethra in order to prevent the loss of urine when the bladder was stimulated during the experiment. Secondly, shear the ventral hair, and snip an incision about 3-5cm above the pubic symphysis along the linea alba abdominis, move out the bladder. Then choose the blood vessels less place on the bladder top, using three hemostatic forceps clamp it, cut the bladder open. Insert the cannular into the bladder immediately and then ligature. Observation item Record the normal urine volume for five minutes, as normal control. The effect of the saline on the urine volume: Inject 20ml saline(38℃)into the vein in the ear margin of the rabbit within 1 minute. Observe the urine volume changes and record. The effect of the norepinephrine on the urine volume: Inject 1:10000 norepinephrine (0.3ml)into the ear margin of the rabbit. Observe the urine volume changes and record. The effect of glucose on the urine volume :Firstly, collect 2drops of urine to test glucose in urine as normal with a qualitative test paper. Then 20% glucose (1.5ml/kg)was injected into the vein in the rabbit’s ear ,and observe the urine volume changes and record.2drops of urine was collected to test glucose in urine qualitatively every 1 or 2 minutes. The effect on the urine volume when the periphe