杂色曲霉素对体外培养人支气管上皮细胞(BEAS-2B)细胞周期影响的研究的中期报告.docxVIP

杂色曲霉素对体外培养人支气管上皮细胞(BEAS-2B)细胞周期影响的研究的中期报告 摘要： 目的：本研究旨在探究杂色曲霉素对体外培养人支气管上皮细胞(BEAS-2B)细胞周期的影响。 方法：采用细胞培养技术，将BEAS-2B细胞分为4组，杂色曲霉素处理组（0.1ug/mL，0.5ug/mL，1ug/mL），对照组，共培养72小时，用流式细胞术测定细胞周期。 结果：与对照组相比，杂色曲霉素处理组BEAS-2B细胞的G0/G1期细胞比例明显升高，而S期和G2/M期细胞比例明显降低，且剂量依赖性显著(P0.05)。同时，BEAS-2B细胞总增殖率呈剂量依赖性表现，杂色曲霉素浓度越高，细胞增殖率越低（P0.05）。 结论：杂色曲霉素可以影响BEAS-2B细胞周期，抑制其增殖，具有一定的抗增殖作用。但其作用机制还需进一步研究。 关键词：杂色曲霉素；支气管上皮细胞；细胞周期；增殖 Mid-term Report on the Effect of Kasugamycin on Cell Cycle of Human Bronchial Epithelial cells (BEAS-2B) in vitro Abstract: Objective: This study aims to investigate the effect of kasugamycin on the cell cycle of human bronchial epithelial cells (BEAS-2B) in vitro. Methods: BEAS-2B cells were divided into four groups, including kasugamycin-treated groups (0.1ug/mL, 0.5ug/mL, 1ug/mL) and control group. Cells were cultured for 72 hours, and the cell cycle was measured by flow cytometry analysis. Results: Compared with the control group, the proportion of G0/G1 phase cells in the kasugamycin-treated group was significantly increased, while the proportion of S and G2/M phase cells was significantly decreased in a dose-dependent manner (P0.05). Meanwhile, the total proliferation rate of BEAS-2B cells showed a dose-dependent decrease, and the higher the concentration of kasugamycin, the lower the cell proliferation rate (P0.05). Conclusion: Kasugamycin can affect the cell cycle of BEAS-2B cells and inhibit their proliferation, indicating a certain anti-proliferative effect. However, its mechanism of action needs to be further studied. Keywords: Kasugamycin; Bronchial epithelial cells; Cell cycle; Proliferation