MBR中活性污泥共代谢降解雌激素机理研究的中期报告.docxVIP

MBR中活性污泥共代谢降解雌激素机理研究的中期报告 摘要： 本研究旨在探究MBR中活性污泥共代谢降解雌激素的机理。该研究采用室内试验的方式，以厌氧-好氧MBR工艺为研究对象，运用荧光定量PCR技术对共代谢菌群的变化进行监测，使用高效液相色谱法对水样中的雌二醇、雌三醇等雌激素类物质进行测定。 研究结果表明，MBR中活性污泥共代谢降解雌激素的机理主要包括匿名菌、普通污泥微生物和泥球菌共同作用，其中泥球菌起到了关键作用。通过对MBR进气流速、污水进水COD、污泥浓度等参数的调整，优化MBR生态环境，可以提高共代谢降解雌激素的效果。此外，MBR中活性污泥对不同种类的雌激素表现出不同的代谢能力，其中雌二醇的降解率最高。 关键词：MBR；共代谢降解；雌激素；机理；优化。 Abstract: This study aimed to explore the mechanism of co-metabolic degradation of estrogen by using activated sludge in MBR. An indoor experiment was conducted, and the anaerobic-aerobic MBR process was used as the object of research. The changes in co-metabolic bacterial communities were monitored by using fluorescence quantitative PCR technology, and the estrogen compounds such as estradiol and estriol in water samples were determined by using high-performance liquid chromatography. The results showed that the mechanism of co-metabolic degradation of estrogen by activated sludge in MBR mainly included the synergistic effect of anonymous bacteria, common sludge microorganisms, and filamentous bacteria, in which filamentous bacteria played a key role. By adjusting the parameters such as the MBR inlet air velocity, COD of sewage inlet, and sludge concentration, the ecological environment of MBR can be optimized to improve the co-metabolic degradation effect of estrogen. In addition, the activated sludge in MBR exhibited different metabolic capacities for different types of estrogen, and the degradation rate of estradiol was the highest. Keywords: MBR; co-metabolic degradation; estrogen; mechanism; optimization.