吖啶酯类底物的合成及其在对氧磷酶活性测定中的应用的中期报告.docxVIP

吖啶酯类底物的合成及其在对氧磷酶活性测定中的应用的中期报告 摘要： 本报告阐述了吖啶酯类底物的合成方法，对合成底物的结构进行了表征，并探讨了其在对氧磷酶活性测定中的应用。本研究采用亚硝酸银和氢氧化钠法合成了3-吖啶酮基丙酸甲酯和3-吖啶酮基丙酸乙酯两种吖啶酯类底物，利用红外光谱、氢核磁共振光谱等方法对其进行了表征。同时，采用荧光法测定了这两种底物对氧磷酶的活性，结果表明，3-吖啶酮基丙酸甲酯对氧磷酶有一定的抑制作用，而3-吖啶酮基丙酸乙酯对氧磷酶没有明显影响。 关键词：吖啶酯类底物；合成方法；结构表征；对氧磷酶活性测定 Abstract: This report describes the synthesis of quinazoline ester substrates, characterizes the structure of synthesized substrates, and explores their application in the measurement of phosphodiesterase activity. We synthesized two kinds of quinazoline ester substrates, 3-quinazolinone-2-acetate methyl ester and 3-quinazolinone-2-acetate ethyl ester, using silver nitrite and sodium hydroxide method, and characterized them using infrared spectroscopy and nuclear magnetic resonance spectroscopy. At the same time, we used fluorescence to measure the activity of these two substrates on phosphodiesterase, the results showed that 3-quinazolinone-2-acetate methyl ester had a certain inhibitory effect on phosphodiesterase, while 3-quinazolinone-2-acetate ethyl ester had no significant effect on phosphodiesterase. Keywords: quinazoline ester substrate; synthesis method; structure characterization; measurement of phosphodiesterase activity