沙门菌反向线性探针杂交检测方法的建立及应用的中期报告.docxVIP

沙门菌反向线性探针杂交检测方法的建立及应用的中期报告 Abstract：Salmonella is a common foodborne pathogen that causes significant economic losses and public health concerns. The establishment of a reverse line blot hybridization method for the detection of Salmonella can improve the efficiency and accuracy of pathogen detection. In this study, we optimized the amplification and labeling system of Salmonella target genes, established a reverse line blot hybridization system, and evaluated its specificity and sensitivity. The results showed that the detection limit of the established method was 5 × 10^2 CFU/mL, and the spe