DNA甲基化检测方法-.pptxVIP

DNA甲基化检测方法总览;主要检测途径;Outcome and interpretation of MSAP analysis. Figure shows a schematic representation of banding patterns obtained by digestion of Apoe–/– or WT DNA with HpaII and EcoRI or MspI and EcoRI. Solid and dashed lines indicate present and absent bands, respectively. For clarity, only polymorphisms characterized by bands specifically present in WT DNA are shown. Flanking EcoRI (E) and HpaII/MspI (CCGG) sites are shown. The methylation status of the HpaII/MspI site is also indicated (hmC, hemimethylated cytosine, i.e. methylated on one strand only). Question marks indicate possible