分子生物学学习资料:MB answers 10.docVIP

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AnswerstoWeaverendofchapterqeustions

Chapter10EukaryoticRNAPolymerasesandTheirPromoters

DiagramtheelutionpatternoftheeukaryoticnuclearRNApolymerasesfromDEAE-Sephadexchromatography.Showwhatyouwouldexpectifyouassayedthesamefractionsinthepresenceof1?g/mlof?-amanitin.

Figure10.1illustratestheresultsoftheelutionpatternandseparationofthreepolymerasesusingDEAE-Sephadexchromatography.TheorderofelutionisindicativeofthebindingaffinityofthethreeenzymestotheDEAEresin,withPolIdisplayingtheweakestinteractionandPolIII

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