丹参PPAR-α受体激动效应的体外研究的开题报告.docxVIP

丹参PPAR-α受体激动效应的体外研究的开题报告

Title:InvitrostudyofPPAR-αreceptoractivationeffectofSalviamiltiorrhiza

Introduction:

Salviamiltiorrhiza,alsoknownasDanshen,hasbeenusedintraditionalChinesemedicineforcenturiesasaremedyforvariousdiseases.Itcontainsmanyactivecompounds,includingtanshinonesandsalvianolicacids,thathavebeenfoundtopossessmultiplepharmacologicalproperties,suchasantioxidant,anti-inflammatory,andcardiovascularprotectiveeffects.RecentstudieshavealsodemonstratedthatDanshenhasthepotentialtoactivateperoxisomeproliferator-activatedreceptoralpha(PPAR-α),atranscriptionalfactorinvolvedinlipidmetabolismandinflammation.Therefore,thisstudyaimstoinvestigatethePPAR-αreceptoractivationeffectofDansheninvitro.

Methodology:

1.Cellculture:Humanhepatomacells(HepG2)willbeculturedinDulbeccosModifiedEaglesMedium(DMEM)supplementedwith10%fetalbovineserum,1%penicillin-streptomycin,and1%L-glutamineinahumidifiedincubatorat37℃with5%CO2.

2.Experimentalgroups:HepG2cellswillbedividedintofourgroups:controlgroup,PPAR-αagonistgroup,low-doseDanshengroup,andhigh-doseDanshengroup.

3.Treatment:Cellsinthecontrolgroupwillreceivenotreatment,whilecellsinthePPAR-αagonistgroupwillbetreatedwith10μMofWy14643,aselectivePPAR-αagonist.Cellsinthelow-doseDanshengroupandhigh-doseDanshengroupwillbetreatedwith50μg/mLand100μg/mLofDanshenextract,respectively.

4.MeasurementofPPAR-αactivity:TherelativePPAR-αactivitywillbeassessedusingtheDual-LuciferaseReporterAssaySystem.ThePPAR-αresponsiveelement(PPRE)plasmidandRenillaluciferaseplasmidwillbeco-transfectedintoHepG2cellswithLipofectamine2000,followedbytreatmentwiththerespectiveexperimentalgroup.

5.Dataanalysis:Thedatawillbeanalyzedusingone-wayANOVAfollowedbyTukeysposthoctest.

ExpectedResults:

Weexpecttoobserve