高产虾青素酵母菌株摇瓶发酵条件的优化.docxVIP

高产虾青素酵母菌株摇瓶发酵条件的优化

刘虹;程秀芳;张志焱;谷巍

【摘要】利用单因素及正交试验法对高产虾青素酵母菌株摇瓶发酵条件进行探索.结果表明，碳源以葡萄糖为宜，氮源以硫酸铵为宜，葡萄糖、硫酸铵、磷酸二氢钾、酵母膏和番茄汁最适添加量分别为4%、0.4%、0.4%、0.6%和0.6%,培养基最适初始pH值为5,最适培养温度为221,最适装液量以50mL/500mL(发酵液体积/三角瓶体积).％Inthisstudy,astaxanthin-high-producingstrainsofyeastsofshakeflaskcultureconditionswereoptimizedusingsinglefactorandorthogonal.Theresultsshowedthattheappropriatecarbonandnitrogensourcewereglucoseandammoniumsulfate,theoptimaldosageofglucose,ammoniumsulfate,dipo-tassiumhydrogenphosphate,yeastextractandtomatojuicewere4%、0.4%、0.4%、0.6%and0.6%,