微藻养殖培养基介绍.docxVIP

(NO.1)2XErdschreibersMediumForutexlb2538

Directions

ErdschreibersMediummadewithSupplementedSeawaterinsteadofPasteurizedSeawater.

For3LTotal1.To3Lofpasteurizedsupplementedseawater(60ppt)asepticallyaddeachofthesterilecomponentsintheorderspecified.2.Vigorouslyswirlthecontentsoftheflasktomixthoroughly.3.Storeatrefrigeratortemperature.

1 3L236mL/3L

1 3L

2

36mL/3L

2.3mM0.067mM

2.3mM

0.067mM

BP360-500) 10mL/3L0.7M

Na2HPO47H2O10mL/3l 002M

(SigmaS-9390)10 LM

150mL/3L

3mL/3L6

3mL/3L

SupplementedSeawater

Directions

For1LTotal1.Toapproximately900mLofPasteurizedSeawater,addthefollowingcomponentsintheorderspecifiedwhilestirringcontinuously.2.Bringtotalvolumeto1LwithPasteurizedSeawater.

1 900mLNaCl(Fisher2S271-500)23g/L393.8mM

1 900mL

NaCl(Fisher

2S271-500)

23g/L

393.8mM

24.6mM28.2mM3MgCl26H2O 5g/L

24.6mM

28.2mM

(FisherM33)

4Na2SO4(SigmaS4g/L

6264)

a)PasteurizedSeawater

Directions

Mostsalt-watermediausedbyUTEXincludesnaturalseawatercollectedoff-shorefromPortAransas,TexasintheGulfofMexico.Seawaterhavingasalinityofatleast30pptiscollectedandpre-filtered,thenstoredundisturbedwithin

polyethylenecarboysatambienttemperature.

Seawaterisdilutedto30pptwithdoubledistilledwaterimmediatelypriortopasteurization.Athree-literbatchofseawaterat30pptina4-literErlenmeyerflaskiscoveredwithasmallinvertedglasspetriplateandaninverted250-mlbeaker,thenpasteurizedinasteamerfor45minutes.Thepasteurizedcontentoftheflaskisallowedtocoolandleftundisturbedatambienttemperatureforapproximately24hr.Itisthenagainsteam-pasteurizedfor45min.,asonthepreviousday.Aftertheflaskcoolsthesecondtime,theinverted-petri-platelidissealedinplacewithParafilmandtheflaskisstoredatrefrigeratortemperatureuntilitisusedtoprepareculturemedium.Thisp