ASTM E1470-92(1998)美国材料与试验协会标准.pdfVIP

Designation:E1470–92(Reapproved1998)

AMERICANSOCIETYFORTESTINGANDMATERIALS

100BarrHarborDr.,WestConshohocken,PA19428

ReprintedfromtheAnnualBookofASTMStandards.CopyrightASTM

StandardTestMethodfor

CharacterizationofProteinsbyElectrophoreticMobility1

ThisstandardisissuedunderthefixeddesignationE1470;thenumberimmediatelyfollowingthedesignationindicatestheyearof

originaladoptionor,inthecaseofrevision,theyearoflastrevision.Anumberinparenthesesindicatestheyearoflastreapproval.A

superscriptepsilon()indicatesaneditorialchangesincethelastrevisionorreapproval.

1.Scopepotentialdistributionsforproteinspecifications,manufacturing

1.1Thistestmethoddescribesaprocedurefordeterminingcontrol,anddevelopmentandresearch.

theelectrophoreticmobilityofproteinsofmolecularweight

4.Apparatus

greaterthan10000Daltons.

1.2ThistestmethodusesautomaticElectrophoreticLight4.1Theapparatusforanalysisconsistsessentiallyofalaser

Scattering(ELS)principlestodeterminetheelectrophoreticlightsource,samplecellforintroducingthesample,power

mobility.supplysource,four256channelspectrumanalyzers,micropro-

2simultaneouslymeasurestheDopplercessors,andcomputerassembly.

1.3Theinstrumen