重症病人的凝血功能障碍.pptVIP

****Toperformaone-stageassay,citrate,whichactsasananticoagulantbybindingcalcium,isaddedtobloodtoinhibitcoagulationuntilassaybegin.Thecitratedbloodismixedandcentrifugedtoisolatetheplasmacomponent.Thromboplastin,arecombinantorcrudeanimaltissueextractthatprovidesphospholipidsandTF,isthenadded,followedbycalcium,whichneutralisesthecitrateandfacilitatesclotformation,andthetimetofibrinclotformationisopticallyassessed.SimilarstepsarefollowedtoperformaPTT,howeverinsteadofusingthromboplastin,asurfaceactivatorsuch