蛋白表达及纯化.pptVIP

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  • 2024-04-07 发布于广东
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**************************AffinityChromatographyMostcommonly-usedchromatographytechniqueCanbeusedonproteinwithnaturalligandsOfteninvolvescovalentattachmentofaffinitytagtoproteinBecauseofuniquetag,providesrapid,specificcleanupinonechromatographystep*Canallowforautomationofproteinpurification第31页,共52页,2024年2月25日,星期天PopularSmallAffinityTagsTagMatrixElutingAgentResiduesSequenceNotesHisNi2+-NTA,Talon,CobaltimidazoleorlowpH5-15HHHHHnativeordenaturing;inexpensiveresinFLAGanti-FLAGantibodylow

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