肺热清解煮散中川贝母煮散工艺研究+最终稿.docxVIP

肺热清解煮散中川贝母煮散工艺研究

【摘要】目的：研究肺热清解煮散中川贝母煮散最佳工艺。方法：先将川贝母粉末用乙醇超声提取，再利用酸性染料比色法制得供试品，用紫外可见分光光度计测定川贝母中西贝母碱的含量。本研究以川贝母为研究对象，以川贝母中西贝母碱的含量作为参考依据，在单因素实验的基础上，以粒度、料液比、时间、提取次数为考察因素，进行正交实验选出川贝母煮散的最佳工艺。结果：研究中，西贝母碱的浓度与吸光度在3.92~39.20μg·mL-1范围内存在线性关系，回归方程为y=0.0258x-0.053，r=0.9996；重复性和稳定性的RSD3%；加样回收率在100.63%~104.38%；最佳工艺为取粒径为0.5mm的川贝母药材粉末，加16倍量的75%乙醇，超声105分钟，超声3次。结论：该方法稳定性和重复性良好、加样回收率良好，且最佳工艺稳定可行，可作为实际生产中川贝母煮散工艺参考。

【关键词】肺热清解煮散；川贝母药材；西贝母碱；紫外可见分光光度法（UV）

StudyonBoilingTechnologyofFritillariaFritillariainFeiReQingJieBoilingPowder

[Abstract]Objective:TostudytheoptimaltechnologyofFritillariafritillariadecoctioninFeiReQingJieBoilingPowder.Methods:ThepowderofFritillariafritillariawasextractedbyultrasonicextractionwithethanol,andthenthetestproductwaspreparedbyaciddyecolorimetry.TheultravioletspectrophotometerwasusedtodeterminethecontentoffritillarineinFritillariafritillaria.Inthisstudy,Fritillariafritillariawastakenastheresearchobject,thecontentoffritillarineinFritillariafritillariawastakenasthereferencebasis,theparticlesize,solid-liquidratio,timeandextractiontimes,basedonthesinglefactortest,weretakenasthefactors,andthebestprocessoffritillariafritillariawasselectedbyorthogonalexperiment.Results:Inthestudy,therewasalinearrelationshipbetweentheconcentrationofkashmirineandabsorbanceintherangeof3.92~39.20μg·mL-1.Theregressionequationwasy=0.0258x-0.053,r=0.9996.RSDSofrepeatabilityandstabilitywerelowerthan3%;Therecoverieswere100.63%-104.38%.TheoptimalprocesswastopulverizeFritillariafritillariaintopowderwithparticlesizeof0.5mm,add75%ethanol16timestheamountforultrasonicextraction,extractiontimeof105mineachtime,extractionfor3times.Conclusion:Themethodhasgoodstabilityandrepeatability,goodrecoveryrate,andtheoptimalprocessisstableandfeasible,whichcanbeusedasreferencefortheboilingpro