（甲基丙烯酸酯-乙二醇二甲基丙烯酸酯）毛细管整体柱的制备研究.docxVIP

聚（甲基丙烯酸酯-乙二醇二甲基丙烯酸酯）毛细管整体柱的制备研究

【摘要】目的：制备聚（甲基丙烯酸酯-乙二醇二甲基丙烯酸酯）毛细管整体柱用于液相色谱分析。方法：以甲基丙烯酸缩水甘油酯（GMA）和2-丙烯酰胺-2-甲基-1-丙磺酸（AMPS）为单体，乙二醇二甲基丙烯酸酯（EDMA）为交联剂，采用热引发自由基聚合的方式制备poly（GMA-co-AMPS-co-EDMA）毛细管整体柱，考察单体与交联剂的用量比、致孔剂的用量比、反应温度等制备条件对柱床形态和渗透性的影响，确定最优条件，通过毛细管液相色谱法对所制备得的毛细管整体柱进行评价。同时，以甲基丙烯酸丁酯（BMA）为单体，乙二醇二甲基丙烯酸酯（EDMA）为交联剂，采用热引发自由基聚合的方式制备poly（BMA-co-EDMA）毛细管整体柱，通过毛细管液相色谱法检测其分离性能。结果：制备poly（GMA-co-AMPS-co-EDMA）毛细管整体柱最佳条件为：功能单体GMA与交联剂EDMA用量比为3:2，致孔剂环己醇与十二醇用量比为10:1，反应温度为50℃，且该整体柱柱体均匀、渗透性好，得到色谱峰拖尾因子为1.04。Poly（BMA-co-EDMA）毛细管整体柱成功分离甲苯和间苯二酚，分离度为2.5。结论：成功制备出poly（GMA-co-AMPS-co-EDMA）和poly（BMA-co-EDMA）毛细管整体柱。

【关键词】毛细管液相色谱；有机聚合物整体柱；聚（甲基丙烯酸酯-乙二醇二甲基丙烯酸酯）整体柱

Preparationofpoly(methacrylate-ethyleneglycoldimethacrylate)capillarymonolithiccolumns

[Abstract]Objective:Preparationofpoly(methacrylate-ethyleneglycoldimethacrylate)forcapillaryliquidchromatography.Methods:Thepoly(GMA-co-AMPS-co-EDMA)monolithiccapillarycolumnwaspreparedbyusingglycidylmethacrylate(GMA)and2-acrylamido-2-methyl-1-propanesulphonicacid(AMPS)asmonomersandethyleneglycoldimethacrylate(EDMA)ascross-linker,andtheeffectofmonomertocross-linkerratio,porogenicagentandreactiontemperatureonthemorphologyandpermeabilityofthecolumnbedwasinvestigated.Theeffectsofthepreparationconditionsonthemorphologyandpermeabilityofthecolumnbed,suchastheratioofmonomertocrosslinker,theratioofporogenicagentandthereactiontemperature,wereinvestigatedtodeterminetheoptimumconditions.Meanwhile,Poly(BMA-co-EDMA)capillarymonolithiccolumnwaspreparedbythermo-initiatedfreeradicalpolymerizationusingbutylmethacrylate(BMA)asmonomerandethyleneglycoldimethacrylate(EDMA)ascross-linker,anditsseparationperformancewasexaminedbycapillaryliquidchromatography.Results:Theoptimumconditionsforthepreparationofpoly(GMA-co-AMPS-co-EDMA)capillarymonolithiccolumn:theratiooffunctionalmonomerGMAtocross-linkerEDMAwas3:2,th