严重急性呼吸综合征冠状病毒主要蛋白酶亚单位关联重要区域和催化作用的关键评估.pdfVIP

THEJOURNALOFBIOLOGICALCHEMISTRYVol.280,No.24,IssueofJune17,pp.22741–22748,2005

©2005byTheAmericanSocietyforBiochemistryandMolecularBiology,Inc.PrintedinU.S.A.

CriticalAssessmentofImportantRegionsintheSubunit

AssociationandCatalyticActionoftheSevereAcute

RespiratorySyndromeCoronavirusMainProtease*

Receivedforpublication,March8,2005,andinrevisedform,April4,2005

Published,JBCPapersinPress,April14,2005,DOI10.1074/jbc.M502556200

Wen-ChiHsu,Hui-ChuanChang,Chi-YuanChou,Pui-JenTsai,Pei-InLin,andGu-GangChang‡

FromtheFacultyofLifeSciences,InstituteofBiochemistry,andStructuralBiologyProgram,

NationalYang-MingUniversity,Taipei112,Taiwan

Thesevereacuterespiratorysyndrome(SARS)coro-length,andtheorganizationissimilartothatofothercorona-

navirus(CoV)mainproteaserepresentsanattractiveviruses.Thereplicasegeneencodestwooverlappingpolypro-

targetforthedevelopmentofnovelanti-SARSagents.teins,polyprotein1a(450kDa)andpolyprotein1ab(750

ThetertiarystructureoftheproteaseconsistsoftwokDa).Thepolyproteinsarecleavedbytheinternallyencoded

distinctfolds.OneistheN-terminalchymotrypsin-likemainprotease(Mpro,3CL),whichisrequiredfortheproduction

foldthatconsistsoftwostructuraldomainsandconsti-ofnewinfectiousviruses.Themainproteaserepresentsan

tutesthecatalyticmachinery;theotheristhe